|
KMID : 0358920010280020207
|
|
Journal of the Korean Academy of Pedodontics
2001 Volume.28 No. 2 p.207 ~ p.218
|
|
|
A effect of calcium hydroxide endodontic materials on the differentiation and the activation of osteoclast
|
|
Jang Eun-Young
Kweon Hoon
Lee Chang-Hee
Lee Chang-Seep
Lee Sang-Ho
|
|
|
Abstract
|
|
|
The purpose of this study was to investigate the direct inhibitory effect or calcium hydroxide materials on differentiation and activation or osteoclast. we used the osteoclast progenitor cells isolated from bone marrow cell of chick embryo tibia and four experimental materials [Ca(OH) 2 powder, MetapatteR, VitapexR, PulpdentR] diluted at 0.1, 0.01, 0.05㎍/ml. There were measured both the lumber of differentiated osteoclast and the area of resorption lacunae. Also, we conducted MTT assay on U2OS osteoblast to examine of cytotoxic effect and obtained following result.
1. Considering the result of the inhibitory effects upon osteoclast differentiation. There were shown a signifitant difference increased in the following ordeer: MetapasteR. Ca(OH) 2 powder, VitapexR. But no significant difference was found in pulpdent group that the number of differentiated osteoclast was increased at all concentration(p<0.05).
2. Among the three experimental groups, that is, Ca(OH) 2 powder, MetapasteR, VitapexR at 0.1㎍/ml dilution that were statistically significant in reduction of the number of differentiated osteoclast. Vitapex group showed significant cytotoxic effect compared to control and another two groups exhibited no significant difference. Also, 0.2% DMSO group was shown statistically siginificant cytotoxicity (p<0.05).
3. Examining pattern and measured area of resorption lacunae in the control and the three experimental
groups, that is Ca(OH) 2 powder, MetapasteR, VitapexR at 0.1㎍/ml dilution, except Ca(OH) 2 powder group, statistically significant differences were found between experimental groups and control group. Also, DMSO group showed statistically significant difference (p<0.05).
From these results, we think that calcium hydroxide is responsible for suppression of hard tissue resorption by a direct inhibition of differentiation and activation of osteoclast.
|
|
|
KEYWORD
|
|
|
Calcium hydroxide, Osteoclast, Differentiation, Activation, inhibitory effect
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|